CHAPTER THREE
3.1 MATERIALS AND METHOD
All subjects used in this study were
apparently healthy students within the age range of [18-30 years]. One hundred
subjects were apparently healthy individual who were students of College of
Health Technology Offa, Kwara State.
3.2 COLLECTION OF SAMPLES
The blood samples were collected by
Various section, 5ml of blood was collected with the aid of needle and syringe
[5ml] into plastic plain bottle provided. The blood sample was centrifuged at
2000v per 5 minute.
3.3 INSTRUMENT AND MATERIALS
The following instruments and
materials were used to perform the test;
i.
Gloves
ii.
Needle and
syringe
iii.
Plain plastic
bottle
iv.
Centrifuge
v.
Stop watch
vi.
Pasteur pipette
vii.
Test tube rack.
3.4 REAGENT AND METHOD
Hepatitis C test strip/rapid test kit, the
test device contains anti hepatitis C particles and hepatitis coated on the
membrane.
PRINCIPLE
The
dinstop one- step strip test device [serum/plasma] is a qualitative internal
flow immunoassay for the detection of HCV is serum or plasma. The membrane is
pre-coated with anti-HCV antibodies on the test line region of the test. During
testing, the serum or plasma specimen reacts with the particle coated with
hepatitis C antibodies on the membrane and generate a coloured line. The
presence of this coloured line in the test region shows that its positive,
while in absence indicates the negative result.
METHOD
1.
The punch was
brought to room temperature before it was opened, the test device was removed
from the sealed punch and it was used as soon as possible.
2.
The test device
was placed on clean and level surface, 60ul of serum was added to the specimen
test device, and then the timer was started. Trampling of air bubble was
avoided in the specimen.
3.
The red lines was
observed after 15minutes to appear
NOTE- A low hepatitis C concentration might result
in a weak line appearing in the test region [t] after an extended period of time;
therefore, the result was checked after 30minutes.
CHAPTER FOUR
4.1 RESULT AND TABLE
One hundred samples were collected
from healthy carriers individual, the results were in table for easy analysis
which shows below in the table form.
Male:- 40 sample was collected and the percentage is
40% and the Degree is 150.0
Female:- 60 Sample
was collected and the percentage is 60% and the Degree is 210.
TABLE 1: Table showing sex of object tested
GENDER
|
FREQUENCY
|
PERCENTAGE
|
DEGREE
|
MALE
|
40
|
40%
|
150.0
|
FEMALE
|
60
|
60%
|
210
|
TOTAL
|
100
|
100%
|
360
|
BAR CHART: Showing sex of the subjected tested
TABLE 2: Table showing age distribution of the subject
tested
AGE GROUP
|
FREQUENCY
|
PERCENTAGE
|
DEGREE
|
15-20
|
38
|
38%
|
140.3
|
21-25
|
52
|
52%
|
213.7
|
26-29
|
10
|
10%
|
6.0
|
BAR CHART: showing age distribution of the subject tested
TABLE 3: showing HCV status of the subject tested
VARIABLOE
|
FREQUENCY
|
PERCENTAGE
|
DEGREE
|
POSITIVE
|
0
|
0%
|
0
|
NEGATIVE
|
100
|
100%
|
360
|
TOTAL
|
100
|
100%
|
360
|
BAR CHAT: showing the subject tested
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